Journal: Molecular Biology of the Cell

Article Title: Sil1, a nucleotide exchange factor for BiP, is not required for antibody assembly or secretion

doi: 10.1091/mbc.E14-09-1392

Figure Lengend Snippet: Sil1 Gt mice respond to T-dependent antigens similarly to age-matched Sil1 WT mice. Sil1 WT (black line) and Sil1 Gt (gray line) mice were challenged with NP 15 -KLH as described, and serum was collected at the indicated intervals. Black arrow indicates the booster immunization. Antigen-specific responses comprised of IgM (A), IgG1 (B), and IgG2b (C) were determined by ELISA. Each point displays the median value. Ten Sil1 WT and Sil1 Gt mice each were immunized and assayed. Error bars indicate interquartile range of values.

Article Snippet: Contrary to our findings, one recent report on an individual with MSS noted decreased levels of serum IgG2 and IgA but not IgM ( Hasegawa et al. , 2014 ).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Molecular Biology of the Cell

Article Title: Sil1, a nucleotide exchange factor for BiP, is not required for antibody assembly or secretion

doi: 10.1091/mbc.E14-09-1392

Figure Lengend Snippet: B lymphoblastoid cell lines produced from individuals with MSS synthesize and secrete IgG antibodies normally. One control and two LBL lines from MSS individuals that produce IgG were pulse labeled with [ 35 S]methionine and cysteine and chased for the indicated times. Cell lysates were immunoprecipitated with anti–γ heavy chain and electrophoresed under reducing (A) or nonreducing (C) conditions. Culture supernatants were similarly immunoprecipitated and analyzed under reducing conditions (B).

Article Snippet: Contrary to our findings, one recent report on an individual with MSS noted decreased levels of serum IgG2 and IgA but not IgM ( Hasegawa et al. , 2014 ).

Techniques: Produced, Control, Labeling, Immunoprecipitation